Microbiology · Board-Style Deep Dive

Animal Farm: The Bugs You Catch From Animals

Every animal in the barnyard hands you a different bacterium. Tap a creature, learn its bug, its gram stain, its buzzword, and its disease. Then walk the cases until the patterns are yours.

Signature interactive

Walk the Barnyard

Each station is one exposure. In Explore, tap an animal to open its dossier. In Name That Bug, read the clue and tap the creature that matches. Labels sit outside the figure so nothing gets lost in the hay.

Tap any animal to open its dossier. The painless black eschar on the sheep is the one students miss most.
From the Attending

Stop memorizing nine random bugs. Memorize the exposure. The animal in the stem hands you the organism before you read a single lab value. Fur and hides means anthrax. Rabbit and tick means tularemia. Flea off a rodent means plague. Find the animal, name the bug.

The dossiers

The Livestock Lineup

One card per bug. Tap to flip for the gram stain, the buzzword, and why that exposure does it. Clinical photos are real and open full screen.

Tap a card to flip. Tap again to close.

Bacillus anthracis
Sheep, wool, hides · Anthrax

Bacillus anthracis

Gram: Gram-positive boxcar rods in chains, spore-forming.
Buzzword: Painless black eschar with a necrotic center and marked non-pitting edema. Inhaled spores cause woolsorters disease with a widened mediastinum.
Why: Spores survive for years in animal wool, hair, and hides.
Treat: Ciprofloxacin or doxycycline.

Francisella tularensis
Rabbit, tick, deerfly · Tularemia

Francisella tularensis

Gram: Tiny gram-negative coccobacillus, intracellular. Needs cysteine-enriched media to grow.
Buzzword: Ulceroglandular disease, a tender ulcer with tender regional nodes. Famously low infectious dose.
Why: Skinning infected rabbits or a tick or deerfly bite.
Treat: Streptomycin or gentamicin.

Yersinia pestis
Flea off a rodent · Plague

Yersinia pestis

Gram: Gram-negative rod with safety-pin bipolar staining on Giemsa or Wright stain.
Buzzword: Painful buboes in bubonic plague. Southwest United States, prairie dogs and rodents.
Why: Flea bite from a rodent reservoir.
Treat: Streptomycin or gentamicin.

Brucella
Unpasteurized dairy · Brucellosis

Brucella

Gram: Gram-negative coccobacillus, intracellular, slow-growing.
Buzzword: Undulant fever that rises and falls, drenching night sweats, and granulomas. Farmers, vets, abattoir workers.
Why: Unpasteurized milk or cheese from cattle and goats.
Treat: Doxycycline plus rifampin.

Pasteurella multocida
Dog or cat bite · Cellulitis

Pasteurella multocida

Gram: Gram-negative coccobacillus, oral flora of cats and dogs.
Buzzword: Rapidly progressive cellulitis with pus within 24 hours of a bite.
Why: Inoculated by the bite itself.
Treat: Amoxicillin-clavulanate.

Bartonella henselae
Cat scratch · Cat-scratch disease

Bartonella henselae

Gram: Gram-negative rod, seen with Warthin-Starry silver stain.
Buzzword: Tender regional lymphadenopathy after a kitten scratch. Bacillary angiomatosis in HIV.
Why: Kitten scratch or flea-contaminated claws.
Treat: Azithromycin.

Leptospira interrogans
Water with rat urine · Weil disease

Leptospira interrogans

Gram: Hook-ended spirochete, too thin for Gram stain. Seen on darkfield.
Buzzword: Flu-like illness with calf myalgias and conjunctival suffusion. Severe form is Weil disease: jaundice plus acute kidney injury. Surfers and triathletes.
Why: Fresh water or mud with animal urine.
Treat: Penicillin or doxycycline.

Coxiella burnetii
Birth fluids, no vector · Q fever

Coxiella burnetii

Gram: Tiny intracellular gram-negative. Highly infectious aerosol, environmentally hardy spore-like form.
Buzzword: No vector, no rash. Atypical pneumonia, and culture-negative endocarditis.
Why: Inhaled birth fluids from parturient sheep, goats, and cattle.
Treat: Doxycycline.

Erysipelothrix rhusiopathiae
Fish, swine, butcher · Erysipeloid

Erysipelothrix rhusiopathiae

Gram: Gram-positive rod.
Buzzword: Erysipeloid, a violaceous, well-demarcated, painful hand lesion in fish handlers and butchers.
Why: Skin inoculation handling fish, meat, or swine.
Treat: Penicillin.

Clinical Images

Real images via Wikimedia Commons. Tap any to expand. These are reference photos with no markers placed on them.

Cutaneous anthrax with black eschar and edema on the skin
📷 Cutaneous anthrax: black eschar · tap to expand
Gram stain of Bacillus anthracis showing boxcar gram-positive rods
📷 B. anthracis: boxcar gram-positive rods · tap to expand
Tularemia ulcer on the skin
📷 Tularemia: ulceroglandular ulcer · tap to expand
Plague bubo in the groin
📷 Plague: painful inguinal bubo · tap to expand
Cat-scratch disease lesion on the hand
📷 Cat-scratch disease: inoculation papule · tap to expand
Darkfield image of Leptospira spirochetes with hooked ends
📷 Leptospira: hook-ended spirochetes · tap to expand
Gram stain of Brucella melitensis coccobacilli
📷 Brucella: gram-negative coccobacilli · tap to expand
The discriminator

The Black Eschar Lock

This is the one students miss. A painless black eschar with a necrotic center, animal fur or hides, and boxcar gram-positive rods is anthrax. Not the vector-borne bugs. Not Staph.

Real cutaneous anthrax eschar
📷 Real photo: cutaneous anthrax · tap to expand
Black necrotic center Painless ulcer rim Non-pitting edema
Your attending drops a chart on the desk and points at the skin photo.
AttendingDrum maker. Works with imported goat hides. Big black sore on his arm. He swears a spider bit him. What is the one feature that ends the argument?
StudentThe spider story?
AttendingForget the spider. Press on the eschar. It does NOT hurt. A spider bite hurts. A staph abscess hurts. This is PAINLESS. Painless plus necrotic plus animal hides is one organism.
StudentBacillus anthracis.
Painless black eschar plus fur or hides plus boxcar gram-positive rods is cutaneous anthrax. Every time.

Run the Fork

A black or ulcerated lesion walks in. Answer each fork before it reveals.

Step 1. There is a skin lesion turning black. You press the eschar. Is the lesion painful or painless, and which way does that point?
Painlessness is the fork. Anthrax edema and lethal toxins kill tissue without the throbbing pain of a Staph abscess. A painful, pus-filled lesion with gram-positive cocci in grape clusters is Staph, never a black eschar.
Step 2. The lesion is painless. The history mentions a job. Which exposure locks in anthrax over tularemia and plague?
Fur and hides is anthrax. Rabbit and tick is tularemia, whose ulcer is tender with tender nodes. Flea and rodent is plague, which gives a painful bubo, not a painless eschar. The exposure sorts the three zoonoses.
Step 3. The Gram stain of the lesion seals it. What confirms Bacillus anthracis?
Boxcar gram-positive rods name anthrax. Cysteine-dependent gram-negative coccobacilli are Francisella, tularemia. Safety-pin bipolar gram-negative rods are Yersinia pestis, plague. The morphology is the final lock.
From the Attending

Three forks, one answer. Painless. Hides. Boxcar rods. Miss the painlessness and you chase a spider or a Staph abscess and you are wrong. Know your clues.

Decision tool

Sort the Barn by Gram Stain

The slide narrows it fast. Tap a bug, then tap the bin where it belongs. Anthrax and Erysipelothrix are the only gram-positives in this barn.

Gram-positive rods
Gram-negative coccobacilli
Gram-negative rods
Spirochete (no Gram)

The Whole Barn on One Card

Self-test: the disease column is blurred. Tap the table to reveal.

Animal or exposureBugGram stainBuzzwordDisease
Wool, hair, hidesBacillus anthracisGram-positive boxcar rods, sporesPainless black eschar; woolsorters diseaseAnthrax
Rabbit, tick, deerflyFrancisella tularensisGram-negative coccobacillus, needs cysteineUlcer plus tender nodes; tiny doseTularemia
Flea off a rodentYersinia pestisGram-negative rod, safety-pin bipolarPainful buboPlague
Unpasteurized dairyBrucellaGram-negative coccobacillus, intracellularUndulant fever, night sweatsBrucellosis
Dog or cat bitePasteurella multocidaGram-negative coccobacillusCellulitis with pus inside 24 hoursBite cellulitis
Cat scratchBartonella henselaeGram-negative rod, silver stainTender regional nodesCat-scratch disease
Water with animal urineLeptospira interrogansHook-ended spirochete, darkfieldCalf myalgias, conjunctival suffusion, WeilLeptospirosis
Livestock birth fluidsCoxiella burnetiiTiny intracellular gram-negativeNo vector, no rash; culture-negative endocarditisQ fever
Fish, swine, butcherErysipelothrix rhusiopathiaeGram-positive rodViolaceous painful hand lesionErysipeloid
Clinical walkthrough

Rounds: Board-Style Vignettes

Eight board-style vignettes. Shuffled, never-repeat, with full explanations for every choice. Cross out and highlight as you read, then walk the reasoning chain.

Medically reviewed by Kaitlyn Cocuzzo, MD and Fatima Ali, DO · Last reviewed June 2026
Sources: CDC, Murray Medical Microbiology, and standard board microbiology references. Clinical photographs via Wikimedia Commons.
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