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Microbiology · Parasitology

Fungi & Parasites

The opportunists and invaders · every high-yield organism in one drill

Opening Challenge

An HIV-positive patient (CD4 count 45) presents with severe headache, fever, and neck stiffness over 5 days. CSF shows elevated opening pressure. India ink prep reveals encapsulated yeast with narrow-based budding. Cryptococcal antigen positive. Organism and correct induction treatment?

A) Aspergillus fumigatus: voriconazole monotherapy
B) Cryptococcus neoformans: liposomal amphotericin B + flucytosine
C) Candida albicans: fluconazole
D) Histoplasma capsulatum: itraconazole
Cryptococcus neoformans, ampho B + flucytosine. India ink shows the clear halo of the polysaccharide capsule. Narrow-based budding distinguishes it from Blastomyces (broad-based). In AIDS with CD4 below 100, Crypto is the most common cause of fungal meningitis. Induction: liposomal ampho B + flucytosine x2 weeks. Consolidation: fluconazole 400mg x8 weeks. Maintenance: fluconazole 200mg indefinitely. High ICP managed with serial LPs. Break it down: CD4 <100 + India ink halo + narrow-based budding + positive cryptococcal antigen = Cryptococcus neoformans meningitis; induction = liposomal ampho B + flucytosine x2 weeks; consolidation = fluconazole 400mg x8 weeks; maintenance = fluconazole 200mg indefinitely; serial LPs manage dangerously high ICP.
01 · Geography

Where the Dimorphics Hide

Geography is the first clue. Tap each region to see which organism dominates.

PACIFIC COAST Low risk SOUTHWEST DESERT Coccidioides AZ, NM, CA desert NORTHWEST GREAT PLAINS OHIO / MISS VALLEY Histoplasma + Blastomyces OH, KY, TN, MS, MO GREAT LAKES Blastomyces SOUTHEAST Low burden NORTHEAST Low burden Coccidioides: SW desert, dust storms, spherules with endospores Histoplasma: Ohio/Miss Valley, bat + bird droppings, spelunking Blastomyces: Great Lakes + SE US, outdoor work, broad-based budding tap a colored region for details
Dimorphic Rule
25°C (cold lab)
MOLD form
Branching hyphae
🩹
37°C (body temp)
YEAST form
Round budding cells
Memory: Mold in the Cold, Yeast in the Beast. Exception: Coccidioides makes spherules with endospores at 37°C (not true yeast).
01 · Organisms

Know the Pattern, Name the Bug

Tap any card to expand. Filter by type or immune status.

Host
01B · High-Yield Villains

The Board Killers

Tap to flip. Geography, clue, host, drug, board move on the back.

Why these 7? These are the organisms that appear in vignettes most. Know the clue that triggers each one before the stem ends.
🦠
Histoplasma
Dimorphic Fungus
Tap to reveal

Geography

Ohio + Mississippi River Valleys · bat/bird droppings, spelunking

Key Clue

Macrophages packed with intracellular oval yeast on Wright/Giemsa stain

Host

HIV CD4 <150 = disseminated · healthy spelunker = self-limited pneumonia

Drug

Itraconazole (mild) · AmB (severe)

Ohio + bat cave + cough = Histo first

🏞
Coccidioides
Dimorphic Fungus
Tap to reveal

Geography

SW United States (Arizona, San Joaquin Valley, desert soil)

Key Clue

Spherules with endospores · NOT true yeast · exception to dimorphic rule

Host

Filipinos + African Americans disseminate · HIV also at risk

Drug

Fluconazole (mild) · AmB (severe/meningitis)

Arizona + dust storm = Cocci

🌿
Blastomyces
Dimorphic Fungus
Tap to reveal

Geography

Great Lakes + SE United States · soil, decaying organic matter

Key Clue

Broad-based budding yeast with double-contoured wall · figure-8 on KOH

Host

Hunters, trappers, campers in Great Lakes · can affect healthy hosts

Drug

Itraconazole (mild) · AmB (severe)

Great Lakes + skin/lung lesion = Blasto

🨐
Cryptococcus
Encapsulated Yeast
Tap to reveal

Geography

Worldwide · pigeon droppings, eucalyptus trees

Key Clue

India ink: clear capsule halo in CSF · narrow-based budding · latex agglutination detects antigen

Host

HIV CD4 <100 gets meningitis · soap bubble basal ganglia lesion

Drug

AmB + Flucytosine (induction) → Fluconazole (maintenance)

HIV + meningitis + clear CSF = India ink STAT

🌼
Aspergillus
Mold (Septate)
Tap to reveal

Microscopy

Septate hyphae, 45-degree branching · halo sign on CT chest

Forms

  • Invasive: neutropenic + CT halo
  • Aspergilloma: fungus ball in old TB cavity
  • ABPA: asthma + eosinophilia

Drug

Voriconazole (invasive) · Itraconazole (ABPA)

Neutropenic + CT halo = Aspergillus until proven otherwise

💀
Mucor / Rhizopus
Mold (Non-Septate)
Tap to reveal

Microscopy

Non-septate hyphae, 90-degree branching · ribbon-like

Key Clue

Black necrotic palate/sinus · angioinvasion = eschar

Host

DKA (most classic) · deferoxamine feeds Mucor (provides iron) · neutropenic

Drug

Amphotericin B + surgical debridement

DKA + black sinus = Mucormycosis STAT

🐈
Toxoplasma
Parasite (Protozoa)
Tap to reveal

Transmission

Cat feces (oocysts) or undercooked meat (bradyzoites) · transplacental (TORCH)

Key Clue

Ring-enhancing brain lesions on MRI · multiple lesions, grey-white junction

Host

HIV CD4 <100 reactivation · fetus: chorioretinitis, hydrocephalus, intracranial calcifications

Drug

Pyrimethamine + Sulfadiazine + folinic acid (NOT folic acid)

Multiple ring lesions + HIV = Toxo vs. CNS lymphoma (single lesion)

The branching rule: Septate + 45-degree = Aspergillus (voriconazole). Non-septate + 90-degree = Mucor (amphotericin B). Wrong drug = wrong answer.
02 · Reference

Overview at a Glance

Geography anchors and helminth cards.

Microscopy shortcut: Aspergillus: septate, 45-degree acute branching. Mucor/Rhizopus: non-septate, 90-degree right-angle branching. This distinction changes treatment completely.
Dimorphic rule: Mold in the cold (25°C), yeast in the beast (37°C). Exception: Coccidioides makes spherules with endospores, not true yeast.
OrganismGeography / SourceUnique ClueAt-Risk Population
HistoplasmaOhio + Mississippi ValleysBat/bird droppings, spelunkingHIV (disseminated), spelunkers
BlastomycesGreat Lakes + SE United StatesSoil, decomposing organic matterHunters, trappers, campers
CoccidioidesSW United States (San Joaquin, Arizona)Desert soil, dust stormsFilipinos, African Americans (disseminated)
ParacoccidioidesLatin America (Brazil)Soil, outdoor workersMales, rural farm workers
SporothrixWorldwide (plants, soil)Rose thorns, sphagnum mossGardeners, florists
CryptococcusWorldwide (pigeon droppings)India ink clear halo, narrow-based buddingHIV CD4 <100, transplant
AspergillusWorldwide (ubiquitous conidia)Halo sign on CT, septate 45-degreeNeutropenic, asthma/CF
Mucor/RhizopusWorldwide (soil, decaying matter)Black palate necrosis, non-septate 90-degreeDKA, deferoxamine use
Helminth drug cheat sheet: Ivermectin (Strongyloides, Onchocerca, Scabies) · Praziquantel (Schistosoma, all tapeworms) · Albendazole (most nematodes, neurocysticercosis + steroids) · DEC (Wuchereria) · Mebendazole (intestinal nematodes alternative)
03 · Elimination Game

Narrow It Down

Use clues to eliminate wrong answers. One organism survives.

Scenario 1 of 3
04 · Quiz

Pattern Recognition

Clinical vignettes. Every wrong answer gets a mechanism-based explanation.

Question 1 of 6 Ring-enhancing brain lesion in an AIDS patient with CD4 count of 68. Multiple lesions concentrated in the basal ganglia. Serum Toxoplasma IgG is positive. Lumbar puncture is deferred due to herniation risk. What is the most appropriate next step?
AStart fluconazole for cryptococcal meningitis
BBrain biopsy to confirm organism before treatment
CEmpiric pyrimethamine + sulfadiazine + leucovorin
DStart voriconazole for CNS Aspergillus
Question 2 of 6 A 55-year-old man with DKA is admitted after 3 days of facial pain and right-sided periorbital swelling. Exam shows black necrotic eschar on the hard palate and right proptosis. CT confirms right orbital invasion. What is the correct immediate treatment?
AStart fluconazole empirically
BLiposomal amphotericin B + urgent surgical debridement
CStart voriconazole for invasive mold
DObserve; this appears to be severe bacterial sinusitis
Question 3 of 6 An HIV-positive patient with CD4 count of 85 presents with 3 weeks of profuse watery, non-bloody diarrhea. Stool acid-fast stain shows small acid-fast oocysts. The patient is not on antiretroviral therapy. What is the most effective intervention?
AMetronidazole
BNitazoxanide
CInitiate antiretroviral therapy for immune reconstitution
DPraziquantel
Question 4 of 6 A 48-year-old man from rural Bolivia presents with progressive dysphagia, constipation, and exertional dyspnea. Echo shows a dilated cardiomyopathy with EF 30%. Barium swallow reveals megaesophagus. He grew up in a home with thatched walls and roofing. What is the vector and causative organism?
AAnopheles mosquito · Plasmodium falciparum
BTriatomine bug · Trypanosoma cruzi (Chagas disease)
CTsetse fly · Trypanosoma brucei
DSandfly · Leishmania donovani
Question 5 of 6 A 34-year-old man with HIV and CD4 count of 48 presents with 3 weeks of progressive dyspnea, non-productive cough, and low-grade fever. Chest X-ray shows bilateral diffuse interstitial infiltrates. LDH is 520 U/L. SpO2 is 88% on room air. What is the correct treatment?
AVoriconazole for invasive mold
BTMP-SMX + prednisone
CLiposomal ampho B + flucytosine for Cryptococcal disease
DAzithromycin for atypical pneumonia
Question 6 of 6 A 28-year-old aid worker returns from 6 months in Guatemala with 2 weeks of bloody diarrhea, crampy abdominal pain, and tenesmus. Stool O&P reveals trophozoites containing ingested red blood cells. LFTs are normal. What is the correct treatment?
ACiprofloxacin for bacterial dysentery
BNitazoxanide
CMetronidazole followed by paromomycin
DTMP-SMX
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